Can I select the root drive and subfolders in it to backup?

When you add to a backup set your root drive, all the subfolders are included automatically. If you don’t want Handy Backup to back up specific files or folders, expand the tree structure and uncheck the boxes near them. For more details, please refer to Step 2: What to Backup in the User Manual.

Note: There is no way to exclude individual files or folders from image backup. When performing image-based backup, the minimal "block" of data that can be backed up is a hard disk partition.